Optimizing the heterologous expression of glutathione transferase
2005 (English)In: Methods of Enzymology, Vol. 401, 254-265 p.Article in journal (Refereed) Published
The heterologous expression of a protein may be enhanced by silent mutations in the coding region of its corresponding DNA. This simple approach has been successfully used for optimized production of a number of glutathione-linked enzymes. For example, the yield of human glutathione transferase M2-2 was elevated by 140-fold in a clone isolated by immunoscreening of a library of plasmids with randomized synonymous codons in the 5'-segment of the region encoding the enzyme.
Place, publisher, year, edition, pages
2005. Vol. 401, 254-265 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-74516OAI: oai:DiVA.org:uu-74516DiVA: diva2:102426