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Efficient enrichment of intact phosphorylated proteins by modified immobilized metal-affinity chromatography
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
2005 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 5, no 18, 4678-4683 p.Article in journal (Refereed) Published
Abstract [en]

Phosphoproteome studies are hampered by the lack of methods which allow a comprehensive and fast analysis of intact phosphoproteins. Here we describe an immobilized metal-affinity chromatography (IMAC)-based technique for the enrichment of phosphorylated proteins, which allows recovery of up to 90% of phosphoproteins. This technique is compatible with 2-DE and can be applied to cultured cells and tissues.

Place, publisher, year, edition, pages
2005. Vol. 5, no 18, 4678-4683 p.
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Medical and Health Sciences Biochemistry and Molecular Biology
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URN: urn:nbn:se:uu:diva-75132DOI: 10.1002/pmic.200500002PubMedID: 16252304OAI: oai:DiVA.org:uu-75132DiVA: diva2:103042
Available from: 2006-01-12 Created: 2006-01-12 Last updated: 2017-12-14Bibliographically approved

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Dubrovska, AnnaSouchelnytskyi, Serhiy

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