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Digital image processing for multiplexing of single molecule detection
Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Centre for Image Analysis. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computerized Image Analysis.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2005 (English)In: Medicinteknikdagarna: Stockholm/Södertälje September 27-28, 2005, 2005Conference paper, Published paper (Other academic)
Abstract [en]

Using padlock and proximity probing techniques, individual molecular identification events are converted to long DNA molecules, carrying repeated sequence motifs used for identification of the detected molecules. We show that identification events can be amplified using rolling circle replication, and randomly attached to a surface for repeated access by identification probes. Repeated hybridization with detection probes carrying fluorescing nano-crystals (quantum dots) of varying spectral properties opens the possibility to search for large numbers of different identification events simultaneously. Methods for digital image processing of the resulting multi-spectral data include spatial as well as spectral data clustering. Spatial data processing includes registration of images from repeated hybridization events as well as delineation of clustered reporter events. Spectral data processing and analysis includes classification of spectral data into groups of either pre-defined or unknown patterns representing different molecular identification events.

Place, publisher, year, edition, pages
2005.
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Computer and Information Science
Identifiers
URN: urn:nbn:se:uu:diva-75228OAI: oai:DiVA.org:uu-75228DiVA: diva2:103138
Note
Details and applications of the single molecule detection were presented by Mats Nilsson during the session on "Föredrag forskning III, Varia".Available from: 2006-01-30 Created: 2006-01-30 Last updated: 2017-02-08Bibliographically approved

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Pinidiyaarachchi, AmalkaGöransson, JennyGonzalez-Rey, CarlosHowell, MathiasMelin, JonasJarvius, JonasNilsson, MatsBengtsson, EwertWählby, Carolina

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Pinidiyaarachchi, AmalkaGöransson, JennyGonzalez-Rey, CarlosHowell, MathiasMelin, JonasJarvius, JonasNilsson, MatsBengtsson, EwertWählby, Carolina
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Centre for Image AnalysisComputerized Image AnalysisDepartment of Genetics and PathologyDepartment of Engineering Sciences
Computer and Information Science

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