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Thermoplastic microfluidic platform for single-molecule detection, cell culture and actuation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences. Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Materials Science.
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2005 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 77, no 22, 7122-30 p.Article in journal (Refereed) Published
Abstract [en]

We have developed a multipurpose microfluidic platform that allows for sensitive fluorescence detection on inexpensive disposable chips. The fabrication scheme involves rapid injection molding of thermoplastics, followed by silica deposition and covalent attachment of an unstructured flexible lid. This combines the virtues of elastomer technology with high-throughput compact disk injection molding. Using this technique, the time to produce 100 chips using a single master can be lowered from more than 1 week by standard PDMS technologies to only a couple hours. The optical properties of the fabricated chips were evaluated by studying individual fluorescence-labeled DNA molecules in a microchannel. Concatemeric DNA molecules were generated through rolling circle replication of circular DNA molecules, which were labeled by hybridization of fluorescence-tagged oligonucleotides. Rolling circle products (RCPs) were detected after as little as 5 min of DNA polymerization, and the RCPs in solution showed no tendency for aggregation. To illustrate the versatility of the platform, we demonstrate two additional applications: The flexible property of the lid was used to create a peristaltic pump generating a flow rate of 9 nL/s. Biocompatibility of the platform was illustrated by culturing Chinese hamster ovary cells for 7 days in the microfluidic channels.

Place, publisher, year, edition, pages
2005. Vol. 77, no 22, 7122-30 p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-75394DOI: 10.1021/ac050916uPubMedID: 16285657OAI: oai:DiVA.org:uu-75394DiVA: diva2:103304
Available from: 2007-01-23 Created: 2007-01-23 Last updated: 2017-12-14Bibliographically approved

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Melin, JonasJohansson, HenrikSöderberg, OlaNikolajeff, FredrikLandegren, UlfNilsson, MatsJarvius, Jonas

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Melin, JonasJohansson, HenrikSöderberg, OlaNikolajeff, FredrikLandegren, UlfNilsson, MatsJarvius, Jonas
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