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Quantification of 10 elements in human cerebrospinal fluid from chronic pain patients with and without spinal cord stimulation
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care.
GE Healthcare Biosci, S-75148 Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care.
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2016 (English)In: Journal of Trace Elements in Medicine and Biology, ISSN 0946-672X, E-ISSN 1878-3252, Vol. 37, p. 1-7Article in journal (Refereed) Published
Abstract [en]

Neuropathic pain affects 1-10% of the general population and is caused by a lesion or disease of the somatosensory nervous system. Spinal cord stimulation (SCS), a method where implanted electrodes stimulate the spinal cord, has been successfully used to treat drug-resistant neuropathic pain, but the mechanism of action is largely unknown. Studies show that SCS changes the protein levels in CSF (cerebrospinal fluid) of pain patients. Several neurological conditions have been shown to alter the elemental composition of CSF. Therefore changes in the levels of ions and trace elements in the CSF may correspond to SCS use. This study used ICP-MS (Inductively coupled plasma mass spectrometry) and ICP-AES (Inductively coupled plasma atomic emission spectroscopy) to quantify 10 elements in CSF from chronic neuropathic pain patients using SCS. The element concentrations in CSF from patients with SCS treatment on/off, were measured. No effect on the element concentrations in CSF from treatment with SCS could be detected. Also, the elemental concentrations in pooled CSF from patients without chronic neuropathic pain was determined and compared to the patients using SCS. The concentration of the elements Ca, Sr, Na, K, P, Mg and Ti were, significantly higher in patients compared to the CSF-control.

Place, publisher, year, edition, pages
2016. Vol. 37, p. 1-7
Keywords [en]
Chronic pain, Spinal cord stimulation, ICP-MS, ICP-AES, Trace elements, CSF
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-304549DOI: 10.1016/j.jtemb.2016.06.003ISI: 000381840800001PubMedID: 27473826OAI: oai:DiVA.org:uu-304549DiVA, id: diva2:1033356
Available from: 2016-10-06 Created: 2016-10-06 Last updated: 2019-10-10Bibliographically approved
In thesis
1. Method development for the analysis of complex samples with inductively coupled plasma mass spectrometry
Open this publication in new window or tab >>Method development for the analysis of complex samples with inductively coupled plasma mass spectrometry
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis the development of methods for handling the problems associated with analyzing trace elements in complex matrixes using inductively coupled plasma mass spectrometry is presented. Trace elements such as Cu, Fe, Se, and Zn, to name a few, do play important roles in different organisms. Therefore it can be of importance to study trace elements in different samples of biological origin. As trace elements are low in abundance, sensitive instrumental techniques such as inductively coupled plasma mass spectrometry (ICP-MS) are required for accurate determination. Due to the complexity of samples with biological origin, careful method development, both regarding the sample preparation and instrumental analysis has to be performed to minimize negative effects on the instrument signal and introduction of interferences.

For example the metal contents of mink livers were analyzed, after bomb digestion to investigate if the metal concentration could be linked to changes in the organ morphology as well as the minks’ environment. Morphological changes and capture locations could be linked to the metals investigated. The investigation of the elemental composition of cerebrospinal fluid from chronic pain patients using spinal cord stimulation electrode treatment on the other hand required less harsh sample treatment. No correlation between the spinal cord stimulation and element concentration could be found, but differences between patients and the control group were presented hinting that chronic pain intrinsically could affect the cerebrospinal fluid metal concentration. Another bodily fluid of interest is saliva and the use of paperpoint sticks as a sampling technique for Ti in saliva was investigated. As Ti is interfered by several components expected to be found in saliva, the use of reaction or collision gas was also investigated to reduce the effects of interferences. Simple leaching of the paperpoint sticks together with complexing the Ti with NH3 as reaction gas was shown to be optimal. Finally, how the selection of internal standard would be affected by the use of reaction and collision gases was also investigated. With collision gas most internal standards worked fine, while for reaction gas internal standard selection was harder. For elements with high ionization energy such as As, Se and Zn the choice of internal standard was very dependent on matrix. While ICP-MS suffers from problems when analyzing samples with complex matrixes many of them can be minimized by proper method development as shown in this thesis.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2019. p. 52
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1865
Keywords
ICP-MS, trace elements, complex matrix.
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:uu:diva-394936 (URN)978-91-513-0772-5 (ISBN)
Public defence
2019-11-29, B41, BMC, Husargatan 3, Uppsala, 13:15 (English)
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Available from: 2019-11-08 Created: 2019-10-10 Last updated: 2019-11-27

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