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A Magnetic Nanobead-Based Read-Out Procedure for Rapid Detection of DNA Molecules
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanoteknologi och funktionella material, Nanotechnology and Functional Materials)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanoteknologi och funktionella material)ORCID iD: 0000-0002-5496-9664
2017 (English)In: Journal of Nanoscience and Nanotechnology, ISSN 1533-4880, E-ISSN 1533-4899, Vol. 17, no 4, 2861-2864 p.Article in journal (Refereed) Published
Abstract [en]

The presented measurement and data analysis procedure reduces the read-out time for the volume-amplified magnetic nanobead detection assay from ~30 min to only 2 min, providing fast, sensitive detection of DNA molecules. The molecular detection and amplification protocol was verified using samples containing rolling circle-amplified DNA products formed from synthetic Vibrio cholerae target DNA, with a limit of detection of 5 pM. The developed read-out method could be used to rapidly identify pathogens in a variety of applications including target screening in hospitals with limited resources, in out-patient settings and in the field.

Place, publisher, year, edition, pages
2017. Vol. 17, no 4, 2861-2864 p.
Keyword [en]
Brownian Relaxation, DNA Macromolecule, Magnetic Nanobeads, Rapid Detection, Rolling Circle Amplification
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-304736DOI: 10.1166/jnn.2017.13877OAI: oai:DiVA.org:uu-304736DiVA: diva2:1033726
Available from: 2016-10-09 Created: 2016-10-09 Last updated: 2017-05-16Bibliographically approved

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Herthnek, David

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Gómez de La Torre, Teresa ZardánHerthnek, DavidStrömme, Maria
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