Neutrophil Receptor Response to Bacterial N-formyl Peptides is Similar in Term Newborn Infants and Adults in Contrast to IL-8.
2016 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083Article in journal (Refereed) Epub ahead of print
We have previously observed that neutrophils from neonates exhibit different migratory responses to intermediate and end-target chemoattractants compared to adults. The aim of the present study was to investigate the effect of the chemoattractants IL-8 (intermediate) and formyl-methionine-leucyl-phenylalanine (fMLP; end-target) on cell surface receptor expression involved in adhesion, migration and granule release of neutrophils from term newborn infants and adults. Heparinized cord blood from 16 healthy term infants delivered by caesarean section and peripheral blood from 17 healthy adults were incubated with 1 μM IL-8 or 0.01 μM fMLP, previously defined as optimal inducers of neutrophil migration. The leukocytes were labelled with antibodies to cell surface receptors (CD11b, CD15S, CD18, CD35, CD44, CD64, CD65, CD88, CD162, CD181 and CD182). Receptor expression was quantified by flow cytometry analysis. Up regulation of CD11b and down regulation of CD88 and CD182 after stimulation with IL-8, was more pronounced in adults than in neonates (p<0.05, p<0.05 and p≤0.001 respectively), whereas fMLP induced changes in receptor expression that were of the same magnitude in neutrophils from neonates as from adults. We observed similar expression of receptors that mediate adhesion, migration, granule activation, and phagocytosis induced by fMLP in neutrophils from neonates and adults. In contrast, differences between neonates and adults, induced by IL-8, suggest that the neutrophil response to intermediate chemoattractants might lead to a compromised infectious response in newborn infants. This article is protected by copyright. All rights reserved.
Place, publisher, year, edition, pages
neutrophils, cell surface molecules, term newborn infants, IL-8, fMLP
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-304757DOI: 10.1111/sji.12477PubMedID: 27606963OAI: oai:DiVA.org:uu-304757DiVA: diva2:1033912