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Comparison of optomagnetic and AC susceptibility readouts in a magnetic nanoparticle agglutination assay for detection of C-reactive protein
Technical University of Denmark.
Technical University of Denmark;Blusense Diagnost, Box 68,Fruebjergvej 3, DK-2100 Copenhagen O, Denmark.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
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2016 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 88, 94-100 p.Article in journal (Refereed) Published
Abstract [en]

There is an increasing need to develop biosensor methods that are highly sensitive and, that can be combined with low-cost consumables. The use of magnetic nanoparticles (MNPs) is attractive because their detection is compatible with low-cost disposables and because application of a magnetic field can be used to accelerate assay kinetics. We present the first study and comparison of the performance of magnetic susceptibility measurements and a newly proposed optomagnetic method. For the comparison we use the C-reactive protein (CRP) induced agglutination of identical samples of 100 nm MNPs conjugated with CRP antibodies. Both methods detect agglutination as a shift to lower frequencies in measurements of the dynamics in response to an applied oscillating magnetic field. The magnetic susceptibility method probes the magnetic response whereas the optomagnetic technique probes the modulation of laser light transmitted through the sample. The two techniques provided highly correlated results upon agglutination when they measure the decrease of the signal from the individual MNPs (turn-off detection strategy), whereas the techniques provided different results, strongly depending on the read-out frequency, when detecting the signal due to MNP agglomerates (turn-on detection strategy). These observations are considered to be caused by differences in the volume-dependence of the magnetic and optical signals from agglomerates. The highest signal from agglomerates was found in the optomagnetic signal at low frequencies.

Place, publisher, year, edition, pages
2016. Vol. 88, 94-100 p.
Keyword [en]
Brownian relaxation, CRP, Agglutination assay, Magnetic beads, Biosensor
National Category
Other Engineering and Technologies
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
URN: urn:nbn:se:uu:diva-305173DOI: 10.1016/j.bios.2016.07.088ISI: 000389112700014PubMedID: 27488263OAI: oai:DiVA.org:uu-305173DiVA: diva2:1034514
Funder
Swedish Research Council Formas, 221-2012-444
Available from: 2016-10-12 Created: 2016-10-12 Last updated: 2017-11-29Bibliographically approved

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Strömberg, MattiasSvedlindh, Peter

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