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Rapid Newcastle Disease Virus Detection based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Technical University of Denmark.
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2016 (English)In: ACS Sensors, ISSN 2379-3694, Vol. 1, no 10, 1228-1234 p.Article in journal (Refereed) Published
Abstract [en]

Rapid and sensitive diagnostic methods based on isothermal amplification are ideal substitutes for PCR in out-of-lab settings. However, there are bottlenecks in terms of establishing low-cost and user-friendly readout methods for isothermal amplification schemes. Combining the high amplification efficiency of loop-mediated isothermal amplification (LAMP) with an optomagnetic' nanoparticle-based readout system, we demonstrate ultrasensitive and rapid detection of Newcastle disease virus RNA. Biotinylated amplicons of LAMP and reverse transcription LAMP (RT-LAMP) bind to streptavidin-coated magnetic nanoparticles (MNPs) resulting in a dramatical increase in the hydrodynamic size of the MNPs. This increase was measured by an optomagnetic readout system and provided quantitative information on the amount of LAMP target sequence. Our assay resulted in a limit of detection of 10 aM of target sequence with a total assay time of 30 min. The assay has also been tested on clinical samples (vaccine and tissue specimens) with a performance comparable to real-time RT-PCR By changing the LAMP primers, this strategy can serve as a general method for the detection of other DNA/RNA targets with high specificity and sensitivity.

Place, publisher, year, edition, pages
2016. Vol. 1, no 10, 1228-1234 p.
Keyword [en]
out-of-lab diagnostics, Newcastle disease virus, loop-mediated isothermal amplification, optomagnetic bioassay, magnetic nanoparticles
National Category
Medical Laboratory and Measurements Technologies Physical Sciences
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
URN: urn:nbn:se:uu:diva-305174DOI: 10.1021/acssensors.6b00379ISI: 000386747600012OAI: oai:DiVA.org:uu-305174DiVA: diva2:1034522
Funder
Swedish Research Council Formas, 221-2012-444Swedish Research Council Formas, 221-2014-574Swedish Research Council Formas, 2011-1692
Available from: 2016-10-12 Created: 2016-10-12 Last updated: 2017-06-16Bibliographically approved

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Tian, BoZardán Gómez de la Torre, TeresaSvedlindh, PeterStrömberg, Mattias

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Tian, BoMa, JingZardán Gómez de la Torre, TeresaSvedlindh, PeterStrömberg, Mattias
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Solid State PhysicsDepartment of Immunology, Genetics and PathologyNanotechnology and Functional Materials
Medical Laboratory and Measurements TechnologiesPhysical Sciences

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