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Chromatographic characterization of substance P endopeptidase in the rat brain reveals affected enzyme activity following heat stress
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2006 (English)In: Biomedical Chromatography, ISSN 0269-3879, Vol. 20, no 1, 77-82 p.Article in journal (Refereed) Published
Abstract [en]

This paper describes a study of substance P endopeptidase (SPE)-like activity in various regions of the brain from male rats subjected to heat stress (HS). The enzyme activity was found to be affected in several brain areas including cerebellum, cerebral cortex, hippocampus, hypothalamus[sol ]thalamus and the spinal cord following HS. Significant increases in SPE activity were observed in, for example, hippocampus and the spinal cord. SPE-containing extracts from hippocampus were pooled and subsequently purified by size exclusion chromatography (using a Superdex® 75 HR column) and by anion-exchange chromatography (using Resource Q® column). The gel permeation chromatography separated the SPE-like activity into two fractions, one of which was suggested to be identical to neutral endopeptidase owing to its molecular size and inhibitory profile. The other active enzyme fraction behaved in conformity with SPE, previously identified in human cerebrospinal fluid. The activity of the purified fraction of these two enzymes was found to be increased (27%) in HS-treated animals.

Place, publisher, year, edition, pages
2006. Vol. 20, no 1, 77-82 p.
Keyword [en]
substance P, substance P endopeptidase, heat stress, purification, HPLC, rat brain, hippocampus
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-75667DOI: 10.1002/bmc.531PubMedID: 15954166OAI: oai:DiVA.org:uu-75667DiVA: diva2:103578
Available from: 2006-02-14 Created: 2006-02-14 Last updated: 2010-05-26Bibliographically approved

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Nyberg, Fred

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