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Approaches and Limits for Accurate Mass Characterization of Large Biomolecules
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Radiation Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Radiation Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Radiation Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Radiation Sciences.
1995 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 67, no 20, 3793-3798 p.3793-3798 p.Article in journal (Refereed) Published
Abstract [en]

The use of the average mass for mass characterization oflarge biomolecules is examined in light of the latestachievements in mass spectrometry, and factors affectingthe accuracy of both theoretical calculation and experimentaldetermination are analyzed. It is concluded that,in practice, the accuracy of average mass measurementsis limited to f O . l Da for molecular masses below 10 000Da and to 10 ppm for masses above that value. Inherentproperties of the isotopic distributions lead to a systematicunderestimation of the average mass during the measurements.The procedure proposed earlier (Zubarev, R A.Int. J. Mass. Spectrom. Ion Processes 1991,107,17-27) in order to correct for this effect is now extended tothe case of multiply-charged ions and their use for massscale calibration. A formula is derived for the relationshipbetween mass accuracy and both the instrumental resolvingpower and molecular ion peak statistics. Monoisotopicmass measurements are recommended to be usedwhenever possible. As a complement to that, otheradditive quantities, such as the ratio of intensities of theh t is otopic peak to the monoisotopic peak, can beemployed.

Place, publisher, year, edition, pages
1995. Vol. 67, no 20, 3793-3798 p.3793-3798 p.
Keyword [en]
magnetic-sector instrument, electrospray-ionization, isotopic distributions, molecular-weight, spectrometry, proteins, resolution, peptides, avarage
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-75850DOI: 10.1021/ac00116a028OAI: oai:DiVA.org:uu-75850DiVA: diva2:103761
Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2017-12-14Bibliographically approved

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