uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
In vitro interactions between insulin-producing beta cells and embryonic dorsal root ganglia.
Uppsala University, Medicinska vetenskapsområdet, Faculty of Medicine, Department of Neuroscience. Neuroanatomi.
Department of Medical Cell Biology. Medical Cell Biology. (Arne Andersson)
2005 (English)In: Pancreas, Vol. 31, no 4, 380-384 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES: It has been suggested that the autoimmune assault in type 1 diabetes is not restricted to islet beta cells but also encompasses intraislet nervous structures. Thus, in addition to modulating islet endocrine function, the nerves may also play a direct pathogenic role in diabetes. A major problem in determining the role of neurons in islet function is to distinguish specific neural effects from those mediated through the vascular system, extrinsic hormones, and/or nutritional factors. The aim of this study was to develop an in vitro system that will enable studies on communication between 1 particular type of neuron and islet cell. METHODS: To achieve this, we cocultured rat islets and rat embryonic dorsal root ganglia (DRG) in a 3-dimensional roller-tube system for 1 to 4 weeks. RESULTS: We found a distinct glucose-induced insulin response throughout the culture period. This response was lower compared with islets

cultured alone. DRGs survived better when they were cocultured with islet cells. CONCLUSIONS: The roller-tube coculture system provides a novel in vitro system for exploring the interaction between different subpopulations of neurons and pancreatic beta cells. Coculture with DRG neurons reduced glucose-induced

insulin release from beta cells, indicating that sensory nerve activity inhibits the insulin response.

Place, publisher, year, edition, pages
2005. Vol. 31, no 4, 380-384 p.
URN: urn:nbn:se:uu:diva-76081PubMedID: 16258374OAI: oai:DiVA.org:uu-76081DiVA: diva2:103992
Available from: 2006-02-23 Created: 2006-02-23 Last updated: 2011-01-11

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Kozlova, ElenaJansson, Leif
By organisation
Department of NeuroscienceDepartment of Medical Cell Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 382 hits
ReferencesLink to record
Permanent link

Direct link