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Versican accumulates in vascular lesions in pulmonary arterial hypertension
Karolinska Inst, Dept Mol Med & Surg, Stockholm, Sweden..
Benaroya Res Inst Virginia Mason, Matrix Biol Program, Seattle, WA USA..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Benaroya Res Inst Virginia Mason, Matrix Biol Program, Seattle, WA USA..
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2016 (English)In: PULMONARY CIRCULATION, ISSN 2045-8932, Vol. 6, no 3, 347-359 p.Article in journal (Refereed) Published
Abstract [en]

Pulmonary arterial hypertension (PAH) is a lethal condition for which there is no effective curative pharmacotherapy. PAH is characterized by vasoconstriction, wall thickening of pulmonary arteries, and increased vascular resistance. Versican is a chondroitin sulfate proteoglycan in the vascular extracellular matrix that accumulates following vascular injury and promotes smooth-muscle cell proliferation in systemic arteries. Here, we investigated whether versican may play a similar role in PAH. Paraffin-embedded lung sections from patients who underwent lung transplantation to treat PAH were used for immunohistochemistry. The etiologies of PAH in the subjects involved in this study were idiopathic PAH, scleroderma, and congenital heart disease (atrial septal defect) with left-to-right shunt. Independent of the underlying etiology, increased versican immunostaining was observed in areas of medial thickening, in neointima, and in plexiform lesions. Western blot of lung tissue lysates confirmed accumulation of versican in patients with PAH. Double staining for versican and CD45 showed only occasional colocalization in neointima of high-grade lesions and plexiform lesions. In vitro, metabolic labeling with [S-35] sulfate showed that human pulmonary artery smooth-muscle cells (hPASMCs) produce mainly chondroitin sulfate glycosaminoglycans. In addition, hypoxia, but not cyclic stretch, was demonstrated to increase both versican messenger RNA expression and protein synthesis by hPASMCs. Versican accumulates in vascular lesions of PAH, and the amount of versican correlates more with lesion severity than with underlying etiology or inflammation. Hypoxia is a possible regulator of versican accumulation, which may promote proliferation of pulmonary smooth-muscle cells and vascular remodeling in PAH.

Place, publisher, year, edition, pages
2016. Vol. 6, no 3, 347-359 p.
Keyword [en]
pulmonary arterial hypertension, versican, vascular smooth-muscle cell, hypoxia, mechanical strain
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-305531DOI: 10.1086/686994ISI: 000383018300012PubMedID: 27683612OAI: oai:DiVA.org:uu-305531DiVA: diva2:1040163
Funder
Swedish Heart Lung Foundation
Available from: 2016-10-26 Created: 2016-10-19 Last updated: 2016-10-26Bibliographically approved

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Eriksson, IngerCao, XiaofangJohansson, StaffanKjellen, Lena
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