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Single Microgels in Core-Shell Equilibrium: A Novel Method for Limited Volume Studies
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
2016 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 120, no 37, 10030-10042 p.Article in journal (Refereed) Published
Abstract [en]

The interactions of cationic surfactant dodecyl-trimethylammonium bromide and cationic protein cytochrome c with anionic polyacrylate microgels have been investigated in microscopic liquid droplets by means of a micropipette technique at ionic strength 0.01 M and pH 8. Experiments on single microgels in solutions of limited amounts of the surfactant provide the first evidence of microgels in a stable biphasic core shell state with the surfactant partitioned to the shell. Under the same conditions, the protein is found to distribute uniformly in the microgels. Quantitative data in the form of swelling and binding isotherms are presented and compared with literature data for macrogels and with predictions of a recent gel theory. Theory is found to be in semiquantitative agreement with the experiments. The importance of polyion-mediated attractions between the protein molecules is analyzed theoretically and proposed to explain the continuous but highly cooperative binding isotherms.

Place, publisher, year, edition, pages
2016. Vol. 120, no 37, 10030-10042 p.
National Category
Medicinal Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-306261DOI: 10.1021/acs.jpcb.6b06086ISI: 000384034100020PubMedID: 27581555OAI: oai:DiVA.org:uu-306261DiVA: diva2:1040202
Funder
Swedish Research Council, 621-2011-4325
Available from: 2016-10-26 Created: 2016-10-26 Last updated: 2016-10-26Bibliographically approved

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Jidheden, ClaesHansson, Per
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