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Hyper-susceptibility of a fusidic acid-resistant mutant of Salmonella to different classes of antibiotics
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2005 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 247, no 2, 215-220 p.Article in journal (Other academic) Published
Abstract [en]

Fusidic acid resistance (FusR) in Salmonella enterica serovar Typhimurium is caused by mutations in fusA, encoding elongation factor G (EF-G). Pleiotropic phenotypes are observed in FusR mutants. Thus, the fusA1 allele (EF-G P413L) is associated with slow growth rate, reduced ppGpp and RpoS levels, reduced heme levels, and increased sensitivity to oxidative stress. The fusA1–15 allele, (EF-G P413L and T423I) derived from fusA1 in a selection for growth rate compensation, is partially compensated in each of these phenotypic defects but maintains its resistance to fusidic acid. We show here that the fusA1 allele is associated with sensitivity to ultraviolet light and increased susceptibility to the inhibitory action of several unrelated antibiotic classes (β-lactam, fluoroquinolone, aminoglycoside, rifampicin, and chloramphenicol). The fusA1–15 allele, in contrast, is less susceptible to UV and to other antibiotics than fusA1. The hyper-susceptibility to multiple antibiotics associated with fusA1 and fusA1–15 is revealed in a novel growth competition assay at sub-MIC concentrations, but not in a standard MIC assay.

Place, publisher, year, edition, pages
2005. Vol. 247, no 2, 215-220 p.
Keyword [en]
Fusidic acid, Salmonella, fusA, Antibiotic susceptibility, Growth competition
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-76128DOI: 10.1016/j.femsle.2005.05.007PubMedID: 15935566OAI: oai:DiVA.org:uu-76128DiVA: diva2:104039
Available from: 2006-02-24 Created: 2006-02-24 Last updated: 2011-07-18Bibliographically approved

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Hughes, Diarmaid

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