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Fabrication of rnacroporous cement scaffolds using PEG particles: In vitro evaluation with induced pluripotent stem cell-derived mesenchymal progenitors
New York Stem Cell Fdn, Res Inst, 1995 Broadway, New York, NY 10023 USA..
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
New York Stem Cell Fdn, Res Inst, 1995 Broadway, New York, NY 10023 USA..
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2016 (English)In: Materials science & engineering. C, biomimetic materials, sensors and systems, ISSN 0928-4931, E-ISSN 1873-0191, Vol. 69, 640-652 p.Article in journal (Refereed) Published
Abstract [en]

Calcium phosphate cements (CPCs) have been extensively used in reconstructive dentistry and orthopedics, but it is only recently that CPCs have been combined with stem cells to engineer biological substitutes with enhanced healing potential. In the present study, macroporous CPC scaffolds with defined composition were fabricated using an easily reproduced synthesis method, with minimal fabrication and processing steps. Scaffold pore size and porosity, essential for cell infiltration and tissue ingrowth, were tuned by varying the content and size of polyethylene glycol (PEG) particles, resulting in 9 groups with different architectural features. The scaffolds were characterized for chemical composition, porosity and mechanical properties, then tested in vitro with human mesenchymal progenitors derived from induced pluripotent stem cells (iPSC-MPs). Biomimetic decellularized bone scaffolds were used as reference material in this study. Our manufacturing process resulted in the formation of macroporous monetite scaffolds with no residual traces of PEG. The size and content of PEG particles was found to affect scaffold porosity, and thus mechanical properties. Irrespective of pore size and porosity, the CPC scaffolds fabricated in this study supported adhesion and viability of human iPSC-MPs similarly to decellularized bone scaffolds. However, the architectural features of the scaffolds were found to affect the expression of bone specific genes, suggesting that specific scaffold groups could be more suitable to direct human iPSC-MPs in vitro toward an osteoblastic phenotype. Our simplistic fabrication method allows rapid, inexpensive and reproducible construction of macroporous CPC scaffolds with tunable architecture for potential use in dental and orthopedic applications.

Place, publisher, year, edition, pages
2016. Vol. 69, 640-652 p.
Keyword [en]
Bioinaterial scaffold, Calcium phosphate cement, Macroporosity, Mesenchymal progenitors, Induced pluripotent stem cells, Tissue engineering
National Category
Materials Engineering
Identifiers
URN: urn:nbn:se:uu:diva-306232DOI: 10.1016/j.msec.2016.06.075ISI: 000383930900076PubMedID: 27612757OAI: oai:DiVA.org:uu-306232DiVA: diva2:1040414
Funder
EU, FP7, Seventh Framework Programme, 262948
Available from: 2016-10-27 Created: 2016-10-26 Last updated: 2016-10-27Bibliographically approved

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Palmer, MichaelÖhman, CarolineEngqvist, Håkan
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