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LexA, a transcription regulator binding in the promoter region of the bidirectional hydrogenase in the cyanobacterium Synechocystis sp. PCC 6803
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Department of Evolution, Genomics and Systematics. Fysiologisk botanik. (Cyano)
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Department of Evolution, Genomics and Systematics. Fysiologisk botanik. (Cyano)
2005 (English)In: FEMS Microbiology Letters, Vol. 251, 59-66 p.Article in journal (Other (popular scientific, debate etc.)) Published
Abstract [en]

The unicellular cyanobacterium Synechocystis PCC 6803 contains a single pentameric bidirectional hydrogenase encoded by hox-EFUYH. Transcriptional experiments demonstrated that the five hox genes are a part of a single transcript together with three ORFs with unknown functions. The transcript start point was localized by 5´RACE to 168bp upstream the hoxE ATG start codon. DNA affinity assays demonstrated a specific interaction between the hox regulatory promoter region and a protein which, using mas spectrometry, was identified to be LexA. Overexpressed His-tagged Synechocystis LexA and EMSA showed a specific binding to the promoter region of the hox operon. Increasing concentrations of the purified LexA resulted in two retarded LexA-DNA complexes, in agreement with the presence of two putative LexA binding sites upstream the determined TSP.

Place, publisher, year, edition, pages
2005. Vol. 251, 59-66 p.
Keyword [en]
Synechocystis, bidirectional hydrogenase, hox, transcription regulator, LexA
Identifiers
URN: urn:nbn:se:uu:diva-76337DOI: doi:10.1016/j.femsle.2005.07.024OAI: oai:DiVA.org:uu-76337DiVA: diva2:104249
Available from: 2006-03-02 Created: 2006-03-02 Last updated: 2011-01-11

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