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Microsatellite genotyping of DNA isolated from claws left on tanned carnivore hides.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Department of Evolution, Genomics and Systematics. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Department of Evolution, Genomics and Systematics, Evolutionary Biology. Evolutionsbiologi. (Conservation Genetics)
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Department of Evolution, Genomics and Systematics. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Department of Evolution, Genomics and Systematics, Evolutionary Biology. Evolutionsbiologi.
2005 (English)In: Int J Legal Med, ISSN 0937-9827, Vol. 119, no 6, 370-3 p.Article in journal (Refereed) Published
Abstract [en]

Tanned hides, a common form of preservation of mammalian specimens, are usually resistant to DNA analysis. However, we show that DNA isolated from the pulp of claws of tanned hides amplifies well for microsatellite markers. For eight wolverine and eight lynx hides tanned 5-20 years ago, 93-98% of replicate amplifications gave distinct PCR products. Genotypes obtained in analysis of tissue samples of the same individuals were in all cases in agreement with those obtained by analysis of claws. We thus conclude that the use of claws from tanned hides offers new possibilities to genetic studies of preserved mammalian specimens, for instance, in the monitoring of illegal trade.

Place, publisher, year, edition, pages
2005. Vol. 119, no 6, 370-3 p.
National Category
Genetics
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URN: urn:nbn:se:uu:diva-76393PubMedID: 15690184OAI: oai:DiVA.org:uu-76393DiVA: diva2:104305
Available from: 2006-03-02 Created: 2006-03-02 Last updated: 2011-01-11

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Hedmark, EvaEllegren, Hans

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