uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Hydrogels bearing bioengineered mimetic embryonic microenvironments for tumor reversion
Harbin Inst Technol, Bio X Ctr, Sch Life Sci & Technol, Harbin 150080, Peoples R China..
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry. Harbin Inst Technol, Bio X Ctr, Sch Life Sci & Technol, Harbin 150080, Peoples R China..
Harbin Inst Technol, Bio X Ctr, Sch Life Sci & Technol, Harbin 150080, Peoples R China..
Harbin Inst Technol, Bio X Ctr, Sch Life Sci & Technol, Harbin 150080, Peoples R China..
Show others and affiliations
2016 (English)In: Journal of materials chemistry. B, ISSN 2050-750X, E-ISSN 2050-7518, Vol. 4, no 37, 6183-6191 p.Article in journal (Refereed) Published
Abstract [en]

Embryonic microenvironments can reverse the metastatic phenotype of aggressive tumors by inhibiting the Nodal signaling pathway. Here, we hypothesize that embryonic microenvironments can be transplanted for the purpose of oncotherapy. We report the development of an injectable bioactive hydrogel system containing the key antagonists of Nodal signaling-Cripto-1 receptor antibodies (2B11)-for the creation of embryonic microenvironments and the examination of their effect on tumor reversion treatment using a mouse model. Our in vitro results show that the hydrogel system can reduce the mitochondrial membrane potential of MDA-MB-231 and MCF-7, promote cell apoptosis, and reduce the invasive ability of cells. Our in vivo results illustrate that the hydrogel system can significantly inhibit tumor growth in both breast cancer and melanoma tumor-bearing mouse models, as well as transform the cell morphology of melanoma B16 cells to melanin-like cells. Furthermore, the results of the up-regulation of tumor suppressor genes and the down-regulation of oncogenes by high-throughput sequencing confirm that the developed system can also selectively turn on some tumor suppressor genes and turn off certain oncogenes so as to prompt the benign reversion of the tumor phenotype. Taken together, our results demonstrate the injectable biomaterial system is able to create an effective microenvironment for melanoma and breast tumor therapy.

Place, publisher, year, edition, pages
2016. Vol. 4, no 37, 6183-6191 p.
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:uu:diva-305959DOI: 10.1039/c6tb00927aISI: 000384444800003OAI: oai:DiVA.org:uu-305959DiVA: diva2:1043713
Available from: 2016-10-31 Created: 2016-10-24 Last updated: 2016-11-28

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Yan, Hongji
By organisation
Polymer Chemistry
In the same journal
Journal of materials chemistry. B
Biomaterials Science

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 41 hits
ReferencesLink to record
Permanent link

Direct link