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A Rickettsiales symbiont of amoebae with ancient features
Univ Vienna, Dept Microbiol & Ecosyst Sci, Althanstr 14, Vienna, Austria..
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution. Uppsala University, Science for Life Laboratory, SciLifeLab.
Univ Vienna, Dept Microbiol & Ecosyst Sci, Althanstr 14, Vienna, Austria..
Univ Vienna, Dept Microbiol & Ecosyst Sci, Althanstr 14, Vienna, Austria..
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2016 (English)In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 18, no 8, 2326-2342 p.Article in journal (Refereed) Published
Abstract [en]

The Rickettsiae comprise intracellular bacterial symbionts and pathogens infecting diverse eukaryotes. Here, we provide a detailed characterization of CandidatusJidaibacter acanthamoeba', a rickettsial symbiont of Acanthamoeba. The bacterium establishes the infection in its amoeba host within 2h where it replicates within vacuoles. Higher bacterial loads and accelerated spread of infection at elevated temperatures were observed. The infection had a negative impact on host growth rate, although no increased levels of host cell lysis were seen. Phylogenomic analysis identified this bacterium as member of the Midichloriaceae. Its 2.4Mb genome represents the largest among Rickettsiales and is characterized by a moderate degree of pseudogenization and a high coding density. We found an unusually large number of genes encoding proteins with eukaryotic-like domains such as ankyrins, leucine-rich repeats and tetratricopeptide repeats, which likely function in host interaction. There are a total of three divergent, independently acquired type IV secretion systems, and 35 flagellar genes representing the most complete set found in an obligate intracellular Alphaproteobacterium. The deeply branching phylogenetic position of CandidatusJidaibacter acanthamoeba' together with its ancient features place it closely to the rickettsial ancestor and helps to better understand the transition from a free-living to an intracellular lifestyle.

Place, publisher, year, edition, pages
2016. Vol. 18, no 8, 2326-2342 p.
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-306766DOI: 10.1111/1462-2920.12881ISI: 000383388800008PubMedID: 25908022OAI: oai:DiVA.org:uu-306766DiVA: diva2:1044491
Funder
EU, European Research Council, 281633Swedish Research Council, 621-2009-4813
Available from: 2016-11-03 Created: 2016-11-03 Last updated: 2016-11-03Bibliographically approved

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Martijn, JoranEttema, Thijs J. G.
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