Comparison of Flow Cytometry and Image-Based Screening for Cell Cycle Analysis
2016 (English)In: Lecture Notes in Computer Science: Image Analysis and Recognition / [ed] Aurélio Campilho, Fakhri Karray, Springer, 2016, Vol. 9730, 623-630 p.Conference paper (Refereed)
Quantitative cell state measurements can provide a wealth of information about mechanism of action of chemical compounds and gene functionality. Here we present a comparison of cell cycle disruption measurements from commonly used flow cytometry (generating onedimensional signal data) and bioimaging (producing two-dimensional image data). Our results show high correlation between the two approaches indicating that image-based screening can be used as an alternative to flow cytometry. Furthermore, we discuss the benefits of image informatics over conventional single-signal flow cytometry.
Place, publisher, year, edition, pages
Springer, 2016. Vol. 9730, 623-630 p.
Quantitative microscopy, DNA content histogram
Bioinformatics (Computational Biology)
IdentifiersURN: urn:nbn:se:uu:diva-307245DOI: 10.1007/978-3-319-41501-7_70OAI: oai:DiVA.org:uu-307245DiVA: diva2:1045954
13th International Conference, ICIAR 2016, in Memory of Mohamed Kamel, Póvoa de Varzim, Portugal, July 13-15, 2016