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Tissue microarray profiling in human heart failure
Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia..
Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia..
Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia..
KTH, Sch Biotechnol, Royal Inst Technol, Dept Prote,Sci Life Lab, Stockholm, Sweden..
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2016 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 16, no 17, 2319-2326 p.Article in journal (Refereed) Published
Abstract [en]

Tissue MicroArrays (TMAs) are a versatile tool for high-throughput protein screening, allowing qualitative analysis of a large number of samples on a single slide. We have developed a customizable TMA system that uniquely utilizes cryopreserved human cardiac samples from both heart failure and donor patients to produce formalin-fixed paraffin-embedded sections. Confirmatory upstream or downstream molecular studies can then be performed on the same (biobanked) cryopreserved tissue. In a pilot study, we applied our TMAs to screen for the expression of four-and-a-half LIM-domain 2 (FHL2), a member of the four-and-a-half LIM family. This protein has been implicated in the pathogenesis of heart failure in a variety of animal models. While FHL2 is abundant in the heart, not much is known about its expression in human heart failure. For this purpose, we generated an affinity-purified rabbit polyclonal anti-human FHL2 antibody. Our TMAs allowed high-throughput profiling of FHL2 protein using qualitative and semiquantitative immunohistochemistry that proved complementary to Western blot analysis. We demonstrated a significant relative reduction in FHL2 protein expression across different forms of human heart failure.

Place, publisher, year, edition, pages
2016. Vol. 16, no 17, 2319-2326 p.
Keyword [en]
Cardiomyopathy, Human heart failure, Lim proteins, Protein arrays, Proteomic profiling, Tissue microarray
National Category
Cardiac and Cardiovascular Systems Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-307299DOI: 10.1002/pmic.201600135ISI: 000383611400003PubMedID: 27364902OAI: oai:DiVA.org:uu-307299DiVA: diva2:1046021
Funder
Knut and Alice Wallenberg Foundation
Available from: 2016-11-11 Created: 2016-11-11 Last updated: 2016-11-11Bibliographically approved

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Science for Life Laboratory, SciLifeLabDepartment of Immunology, Genetics and Pathology
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