uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Tissue microarray profiling in human heart failure
Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia..
Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia..
Univ Sydney, Bosch Inst, Sydney Med Sch, Dept Anat & Histol, Sydney, NSW, Australia..
KTH, Sch Biotechnol, Royal Inst Technol, Dept Prote,Sci Life Lab, Stockholm, Sweden..
Show others and affiliations
2016 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 16, no 17, 2319-2326 p.Article in journal (Refereed) Published
Abstract [en]

Tissue MicroArrays (TMAs) are a versatile tool for high-throughput protein screening, allowing qualitative analysis of a large number of samples on a single slide. We have developed a customizable TMA system that uniquely utilizes cryopreserved human cardiac samples from both heart failure and donor patients to produce formalin-fixed paraffin-embedded sections. Confirmatory upstream or downstream molecular studies can then be performed on the same (biobanked) cryopreserved tissue. In a pilot study, we applied our TMAs to screen for the expression of four-and-a-half LIM-domain 2 (FHL2), a member of the four-and-a-half LIM family. This protein has been implicated in the pathogenesis of heart failure in a variety of animal models. While FHL2 is abundant in the heart, not much is known about its expression in human heart failure. For this purpose, we generated an affinity-purified rabbit polyclonal anti-human FHL2 antibody. Our TMAs allowed high-throughput profiling of FHL2 protein using qualitative and semiquantitative immunohistochemistry that proved complementary to Western blot analysis. We demonstrated a significant relative reduction in FHL2 protein expression across different forms of human heart failure.

Place, publisher, year, edition, pages
2016. Vol. 16, no 17, 2319-2326 p.
Keyword [en]
Cardiomyopathy, Human heart failure, Lim proteins, Protein arrays, Proteomic profiling, Tissue microarray
National Category
Cardiac and Cardiovascular Systems Cell and Molecular Biology
URN: urn:nbn:se:uu:diva-307299DOI: 10.1002/pmic.201600135ISI: 000383611400003PubMedID: 27364902OAI: oai:DiVA.org:uu-307299DiVA: diva2:1046021
Knut and Alice Wallenberg Foundation
Available from: 2016-11-11 Created: 2016-11-11 Last updated: 2016-11-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Science for Life Laboratory, SciLifeLabDepartment of Immunology, Genetics and Pathology
In the same journal
Cardiac and Cardiovascular SystemsCell and Molecular Biology

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 62 hits
ReferencesLink to record
Permanent link

Direct link