Peptide phage display for probing GST-protein interactions.
2005 (English)In: Methods Enzymol, ISSN 0076-6879, Vol. 401, 354-67 p.Article in journal (Refereed) Published
Phage display is a powerful strategy for identifying protein-peptide interactions. Glutathione transferases (GSTs) play prominent roles in the cellular protection against oxidative stress by catalyzing detoxication reactions. In addition, GSTs seem to act in signaling pathways by means of interaction with other macromolecules such as protein kinases. This chapter describes how the technique of peptide phage display can be used to identify possible partners in GST-protein complexes.
Place, publisher, year, edition, pages
2005. Vol. 401, 354-67 p.
Amino Acid Sequence, Cell Line, Gene Expression Regulation, Genetic Vectors, Glutathione Transferase/genetics/*metabolism, Humans, Isoenzymes/genetics/metabolism, Models; Molecular, Molecular Sequence Data, Peptide Library, Peptides/genetics/*metabolism, Protein Binding, Protein Interaction Mapping, Research Support; Non-U.S. Gov't, Signal Transduction/physiology
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-76696PubMedID: 16399397OAI: oai:DiVA.org:uu-76696DiVA: diva2:104608