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One-step purification of epigallocatechin gallate from crude green tea extracts by isocratic hydrogen bond adsorption chromatography on β-cyclodextrin substituted agarose gel media
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Department of Physical and Analytical Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
2005 (English)In: Journal of Chromatography B, Vol. 824, no 1-2, 323-326 p.Article in journal (Refereed) Published
Abstract [en]

An oligomerized β-cyclodextrin ligand coupled to brominated allyl-group substituted Sepharose HP has been used for the one-step purification of polyphenolic epigallocatechin gallate (EGCG), an important antioxidant, by isocratic hydrogen bond adsorption chromatography. With a sample load of 1.33 mg crude green tea polyphenolic extract per ml column packing and with water/ethanol/acetonitrile (57/30/13, v/v) as the optimum mobile phase, an EGCG purity of about 98% with a recovery of approximate 73% could be achieved by proper peak cutting. After about 10 sample applications, the column performance started to deteriorate but could be regenerated to its original function by cleaning with 0.35 M NaOH.

Place, publisher, year, edition, pages
2005. Vol. 824, no 1-2, 323-326 p.
Keyword [en]
β-Cyclodextrin, Tea polyphenols, EGCG, Catechin
Identifiers
URN: urn:nbn:se:uu:diva-76834OAI: oai:DiVA.org:uu-76834DiVA: diva2:104746
Available from: 2007-02-13 Created: 2007-02-13 Last updated: 2011-01-11

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Janson, Jan-Christer

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