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A new method for analysis of underivatized free beta-methylamino-alanine: Validation and method comparison
Natl Food Agcy, Box 622, SE-75126 Uppsala, Sweden..
Natl Food Agcy, Box 622, SE-75126 Uppsala, Sweden..
Natl Food Agcy, Box 622, SE-75126 Uppsala, Sweden..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry. Natl Vet Inst SVA, Sect Chem Anal, Dept Chem Environm & Feed Hyg, SE-75189 Uppsala, Sweden..
2016 (English)In: Toxicon, ISSN 0041-0101, E-ISSN 1879-3150, Vol. 121, 105-108 p.Article in journal (Refereed) Published
Abstract [en]

A new method was developed for analysis of free beta-Methylamino-alanine (BMAA) in biological matrices. The method is based on direct analysis of the underivatized molecule, using an amide column for separation by Hydrophilic Interaction Liquid Chromatography (HILIC) and detection by tandem mass spectrometry (MS/MS) using a deuterium labeled internal standard. The use of Ultra-High Performance Liquid Chromatography (UHPLC) combined with MS/MS detection allowed for high chromatographic resolution and a low limit of detection (0.025 mu g/g wet weight (ww) in mussels). The method was validated by analyzing spiked blank mussels from the Baltic Sea (0.15-4.4 mu g/g (ww), trueness 99%-105%, RSD 2%-8%). An inter-laboratory comparative analysis of extracts of mussel was performed. The mussels were extracted according to an established protocol for analysis of free BMAA, and the extracts were then analyzed in parallel by the new method and a validated procedure based on detection of BMAA derivatized with dansyl chloride. Both methods detected BMAA in similar concentrations. Thus, derivatization with dansyl chloride did not influence the results compared to direct detection. The new method presents an alternative to the commonly applied derivatization step, and is proved through validation and method comparison to reliably identify and quantify free BMAA at low concentration levels.

Place, publisher, year, edition, pages
2016. Vol. 121, 105-108 p.
Keyword [en]
BMAA, Analysis, Mussels, Interlaboratory comparison, HILIC-UHPLC-MS/MS
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:uu:diva-307743DOI: 10.1016/j.toxicon.2016.08.021ISI: 000385326300013PubMedID: 27592200OAI: oai:DiVA.org:uu-307743DiVA: diva2:1048369
Available from: 2016-11-21 Created: 2016-11-21 Last updated: 2016-11-21Bibliographically approved

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