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Chondrocytes Derived From Mesenchymal Stromal Cells and Induced Pluripotent Cells of Patients With Familial Osteochondritis Dissecans Exhibit an Endoplasmic Reticulum Stress Response and Defective Matrix Assembly
Natl Univ Ireland Galway, Sch Med, Regenerat Med Inst, Galway, Ireland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medicinsk genetik och genomik. Uppsala University, Science for Life Laboratory, SciLifeLab.
Natl Univ Ireland Galway, Sch Med, Regenerat Med Inst, Galway, Ireland..
Lund Univ, Dept Clin Sci Rheumatol & Mol Skeletal Biol, Lund, Sweden..
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2016 (English)In: Stem Cells Translational Medicine, ISSN 2157-6564, E-ISSN 2157-6580, Vol. 5, no 9, 1171-1181 p.Article in journal (Refereed) Published
Abstract [en]

Familial osteochondritis dissecans (FOCD) is an inherited skeletal defect characterized by the development of large cartilage lesions in multiple joints, short stature, and early onset of severe osteoarthritis. It is associated with a heterozygous mutation in the ACAN gene, resulting in a Val-Met replacement in the C-type lectin domain of aggrecan. To understand the cellular pathogenesis of this condition, we studied the chondrogenic differentiation of patient bone marrow mesenchymal stromal cells (BM-MSCs). We also looked at cartilage derived from induced pluripotent stem cells (iPSCs) generated from patient fibroblasts. Our results revealed several characteristics of the differentiated chondrocytes that help to explain the disease phenotype and susceptibility to cartilage injury. First, patient chondrogenic pellets had poor structural integrity but were rich in glycosaminoglycan. Second, it was evident that large amounts of aggrecan accumulated within the endoplasmic reticulum of chondrocytes differentiated from both BM-MSCs and iPSCs. In turn, there was a marked absence of aggrecan in the extracellular matrix. Third, it was evident that matrix synthesis and assembly were globally dysregulated. These results highlight some of the abnormal aspects of chondrogenesis in these patient cells and help to explain the underlying cellular pathology. The results suggest that FOCD is a chondrocyte aggrecanosis with associated matrix dysregulation. The work provides a new in vitro model of osteoarthritis and cartilage degeneration based on the use of iPSCs and highlights how insights into disease phenotype and pathogenesis can be uncovered by studying differentiation of patient stem cells.

Place, publisher, year, edition, pages
2016. Vol. 5, no 9, 1171-1181 p.
Keyword [en]
Osteoarthritis, Familial osteochondritis dissecans, Mesenchymal stromal cells, Induced pluripotent stem cells, Stem cell disease models, Cellular pathology, Aggrecan mutation, Endoplasmic reticulum stress
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-307869DOI: 10.5966/sctm.2015-0384ISI: 000382420500008PubMedID: 27388238OAI: oai:DiVA.org:uu-307869DiVA: diva2:1048901
Funder
EU, European Research Council, 223298
Available from: 2016-11-22 Created: 2016-11-22 Last updated: 2016-11-22Bibliographically approved

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Stattin, Eva-Lena
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Medicinsk genetik och genomikScience for Life Laboratory, SciLifeLab
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