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Direct imaging of elemental distributions in tissue sections by laser ablation mass spectrometry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
TU Wien, Inst Chem Technol & Analyt, Vienna, Austria..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. Fudan Univ, Dept Chem, Inst Biomed Sci, Shanghai, Peoples R China.;Fudan Univ, State Key Lab Mol Engn Polymers, Shanghai, Peoples R China..
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2016 (English)In: Methods, ISSN 1046-2023, E-ISSN 1095-9130, Vol. 104, 86-92 p.Article in journal (Refereed) Published
Abstract [en]

We present a strategy for imaging of elements in biological tissues using laser ablation (LA) mass spectrometry (MS), which was compared to laser ablation inductively coupled plasma (LA-ICP) MS. Both methods were adopted for quantitative imaging of elements in mouse kidney, as well as traumatic brain injury model tissue sections. MS imaging (MSI) employing LA provides quantitative data by comparing signal abundances of sodium from tissues to those obtained by imaging quantitation calibration standards of the target element applied to adjacent control tissue sections. LA-ICP MSI provided quantitative data for several essential elements in both brain and kidney tissue sections using a dried-droplet approach. Both methods were used to image a rat model of traumatic brain injury, revealing accumulations of sodium and calcium in the impact area and its peripheral regions. LA MSI is shown to be a viable option for quantitative imaging of specific elements in biological tissue sections.

Place, publisher, year, edition, pages
2016. Vol. 104, 86-92 p.
Keyword [en]
Brain, Kidney, Elemental imaging, Mass spectrometry, Laser ablation, Inductively coupled plasma
National Category
Radiology, Nuclear Medicine and Medical Imaging Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-308261DOI: 10.1016/j.ymeth.2016.05.021ISI: 000380299300012PubMedID: 27263025OAI: oai:DiVA.org:uu-308261DiVA: diva2:1049325
Funder
Swedish Research Council, 2013-3105; 2014-6215The Swedish Brain FoundationSwedish Foundation for Strategic Research , RIF14-0078
Available from: 2016-11-24 Created: 2016-11-24 Last updated: 2016-11-24Bibliographically approved

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Shariatgorji, MohammadrezaNilsson, AnnaMarklund, NiklasClausen, FredrikKallback, PatrikAndrén, Per E.
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