Salt-Gradient Approach for Regulating Capture-to-Translocation Dynamics of DNA with Nanochannel Sensors
2016 (English)In: ACS SENSORS, ISSN 2379-3694, Vol. 1, no 6, 807-816 p.Article in journal (Refereed) Published
Understanding the physical mechanisms that govern the ion and fluidic transport in salt-concentration-based nanochannel/nanopore systems is essential for the potential applications in bioanalysis. One central challenge is to interpret the observed four-stage change from osmosis to the reverse one with increasing salt gradient. Here we provide a unified model that outlines the intriguing role of two competing factors, the exclusion- and diffusion-induced electrical potentials. We demonstrate theoretically a direction control of a hydrodynamic flow via the salt gradient. Based on this, we also propose a salt-gradient approach for regulating DNA motion in nanochannels that enables voltage-free single-molecule capture with a significantly low translocation speed. The present method would be used as a useful protocol to overcome the key hurdle of tailoring the capture-to-translocation dynamics of polynucleotides for nanopore sequencing.
Place, publisher, year, edition, pages
2016. Vol. 1, no 6, 807-816 p.
salt gradient, DNA manipulating, DNA capture, nanochannel, electrokinetic modeling
Engineering and Technology
IdentifiersURN: urn:nbn:se:uu:diva-308048DOI: 10.1021/acssensors.6b00176ISI: 000385464800026OAI: oai:DiVA.org:uu-308048DiVA: diva2:1049329
FunderSwedish Research Council