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The NudA protein in the gastric pathogen Helicobacter pylori is an ubiquitous and constitutively expressed dinucleoside polyphosphate hydrolase
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2003 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 278, no 14, 12574-12578 p.Article in journal (Other academic) Published
Abstract [en]

The gastric pathogen Helicobacter pylori harbors one Nudix hydrolase, NudA, that belongs to the nucleoside polyphosphate hydrolase subgroup. In this work, the enzymatic activity of purified recombinant NudA protein was analyzed on a number of nucleoside polyphosphates. This predicted 18.6-kDa protein preferably hydrolyzes diadenosine tetraphosphate, Ap(4)A at a k(cat) of 0.15 s(-1) and a K(m) of 80 microm, resulting in an asymmetrical cleavage of the molecule into ATP and AMP. To study the biological role of this enzyme in H. pylori, an insertion mutant was constructed. There was a 2-7-fold decrease in survival of the mutant as compared with the wild type after hydrogen peroxide exposure but no difference in survival after heat shock or in spontaneous mutation frequency. Western blot analyses revealed that NudA is constitutively expressed in H. pylori at different growth stages and during stress, which would indicate that this protein has a housekeeping function. Given that H. pylori is a diverse species and that all the H. pylori strains tested in this study harbor the nudA gene and show protein expression, we consider NudA to be an important enzyme in this bacterium.

Place, publisher, year, edition, pages
2003. Vol. 278, no 14, 12574-12578 p.
Keyword [en]
Bacterial Proteins/genetics/metabolism, Cloning; Molecular, Dinucleoside Phosphates/*metabolism, Gene Expression Regulation; Bacterial, Gene Expression Regulation; Enzymologic, Helicobacter pylori/*enzymology/genetics/growth & development, Hydrolases/genetics/*metabolism, Kinetics, Mutagenesis; Insertional, Oxidative Stress/physiology, Research Support; Non-U.S. Gov't, Substrate Specificity
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Medical and Health Sciences
URN: urn:nbn:se:uu:diva-77052DOI: 10.1074/jbc.M212542200PubMedID: 12551907OAI: oai:DiVA.org:uu-77052DiVA: diva2:104964
Available from: 2006-03-10 Created: 2006-03-10 Last updated: 2011-10-13Bibliographically approved

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Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=12551907&dopt=Citation

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Andersson, Dan IEngstrand, Lars
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