Sequence analysis of the ribosomal internal transcribed spacer DNA of the crayfish parasite Psorospermium haeckeli.
2001 (English)In: Diseases of Aquatic Organisms, ISSN 0177-5103, Vol. 46, no 3, 217-22 p.Article in journal (Refereed) Published
Two morphotypes of the crayfish parasite Psorospermium haeckeli were isolated from 2 crayfish species of different geographical origin. The oval-shaped sporocysts were obtained from the epidermal and connective tissue beneath the carapace of the noble crayfish Astacus astacus fromSweden and Finland. Elongated spores were isolated from the abdominal muscle tissue of the red swamp crayfish Procambarus clarkii from USA. To compare genetic divergence of 2 morphotypes of the parasite, the ribosomal internal transcribed spacer (ITS) DNA (ITS 1 and ITS 2) and the 5.8S rRNAgene were cloned and sequenced. The analysed region is variable in length, with the ribosomal ITS sequence of the European morphotype longer than the North American one. Sequence diversity is found mainly in ITS 1 and ITS 2 regions, and there is 66% and 58% similarity between the 2 morphotypes,respectively. Thus, analysis of the ribosomal ITS DNA suggests that P. haeckeli forms obtained from Europe and North America are genetically diverse, which supports the previously reported morphological characteristics.
Place, publisher, year, edition, pages
2001. Vol. 46, no 3, 217-22 p.
Animals, Astacoidea parasitology, Base Sequence, DNA, Ribosomal chemistry Molecular Sequence Data, Parasites genetics, Polymerase Chain Reaction, veterinary, RNA, , Ribosomal, 5.8S genetics, Research Support, Non-U.S. Gov't, Sequence Alignment/veterinary, Sequence Analysis, DNA
IdentifiersURN: urn:nbn:se:uu:diva-77085PubMedID: 11710556OAI: oai:DiVA.org:uu-77085DiVA: diva2:104997