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Multispacer typing technique for sequence-based typing of Bartonella quintana
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Evolution, Genomics and Systematics, Molecular Evolution.
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2005 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, E-ISSN 1098-660X, Vol. 43, no 1, 41-48 p.Article in journal (Refereed) Published
Abstract [en]

Bartonella quintana is a worldwide fastidious bacterium of the Alphaproteobacteria responsible for bacillary angiomatosis, trench fever, chronic lymphadenopathy, and culture-negative endocarditis. The recent genome sequencing of a B. quintana isolate allowed us to propose a genome-wide sequence-based typing method. To ensure sequence discrimination based on highly polymorphic areas, we amplified and sequenced 34 spacers in a large collection of B. quintana isolates. Six of these exhibited polymorphisms and allowed the characterization of 4 genotypes. However, the strain variants suggested by the noncoding sequences did not correlate with the results of pulsed-field gel electrophoresis (PFGE), which suggested a higher degree of variability. Modification of the PFGE profile of one isolate after nine subcultures confirmed that rearrangement frequencies are high in this species, making PFGE unreliable for epidemiological purposes. The low extent of sequence heterogeneity in the species suggests a recent emergence of this bacterium as a human pathogen. Direct typing of natural samples allowed the identification of a fifth genotype in the DNA extracted from a human body louse collected in Burundi. We have named the typing technique herein described multispacer typing.

Place, publisher, year, edition, pages
2005. Vol. 43, no 1, 41-48 p.
Keyword [en]
Animals, Bacteremia/microbiology, Bacterial Typing Techniques, Bartonella quintana/*classification/genetics/isolation & purification, DNA; Bacterial/analysis, DNA; Ribosomal Spacer/*analysis, Electrophoresis; Gel; Pulsed-Field, Genotype, Heart Valves/microbiology, Humans, Lice/microbiology, Molecular Sequence Data, Research Support; Non-U.S. Gov't, Sequence Analysis; DNA, Trench Fever/microbiology
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-77157DOI: 10.1128/JCM.43.1.41-48.2005PubMedID: 15634949OAI: oai:DiVA.org:uu-77157DiVA: diva2:105069
Available from: 2006-03-13 Created: 2006-03-13 Last updated: 2011-02-18Bibliographically approved

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Lindroos, HilleviAndersson, Siv
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