Measuring temperature in the lens during experimental heat load indirectly as light scattering increase rate
(English)In: Journal of Biomedical Optics, ISSN 1083-3668, E-ISSN 1560-2281Article in journal (Refereed) Accepted
The current study aims to experimentally estimate the temperature in the lens due to heat load indirectly from the measurement of increase rate of temperature-induced light scattering. The lens was extracted from Sprague-Dawley rats and put into a temperature-controlled cuvette filled with balanced salt solution. Altogether, 80 lenses were equally divided on four temperature groups. Each lens was exposed for 5 minutes to temperature depending on group belonging while the intensity of forward light scattering was recorded. The inclination coefficient of light scattering increase at the temperature 37, 40, 43, and 46 ºC was estimated as a CI(0.95), 3.1±0.8, 4.4±0.8, 5.5±0.9 and 7.0±0.8 x10-4 tEDC/s, respectively. The Arrhenius equation implies that the natural logarithm of the inclination coefficient is linearly dependent on the inverse of the temperature. The proportionality constant and the intercept were 9.6±2.4 x103 K and 22.8±7.7. The activation energy was 8.0±2.0 x101 kJ·mol-1. The current experiment implies that if averaging 20 measurements of inclination coefficients in a new experiment at constant heat load, the confidence limits for predicted temperature correspond to ±1.9 °C. With the proportionality constant and the intercept estimated in the current experiment, the in vivo temperature in the lens can be determined retrospectively with sufficient resolution.
forward light scattering; lens; cataract; temperature; Arrhenius equation
Research subject Ophtalmology
IdentifiersURN: urn:nbn:se:uu:diva-308820OAI: oai:DiVA.org:uu-308820DiVA: diva2:1050911