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Bioinformatic Analysis of Genomic and Proteomic Data from Gemmata
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
2016 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Members of the bacterial phylum Planctomycetes have been claimed to have a

compartmentalised cell plan, with cell walls lacking peptidoglycan despite being free-living.

These theories have been challenged in recent years, and the nature of the planctomycete cell

structure is currently under debate. Yet it remains clear that the planctomycete membranes

have unique properties, and are thus likely localisations of evolutional innovation. In this

study, proteomes and genomes of four planctomycete species from the Gemmata/Tuwongella

clade were investigated with the aim to find candidate genes for functional characterisation.

Analysis based on full genome sequencing and mass spectrometry revealed 21 proteins unique

to the Gemmata/Tuwongella clade that were present in the proteomes of all four species. The

gene coding for one of these was found to be organised in an operon, containing an additional

four clade-specific genes, likely related to type II secretion. A planctomycete-specific cell

surface signal peptide previously not seen in Gemmata was identified in all four species, with

proteins found to have the motif indicating that their cell surface has a strong negative charge.

Lastly, the study has revealed evidence suggesting that the planctomycetes have a traditional

gram-negative cell wall, contradicting the previously proposed proteinaceous cell wall model.

Place, publisher, year, edition, pages
2016.
Series
UPTEC X, 16 030
National Category
Evolutionary Biology Bioinformatics and Systems Biology Engineering and Technology
Identifiers
URN: urn:nbn:se:uu:diva-308882OAI: oai:DiVA.org:uu-308882DiVA: diva2:1051136
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2016-12-01 Created: 2016-12-01 Last updated: 2016-12-01Bibliographically approved

Open Access in DiVA

The full text will be freely available from 2018-10-01 10:40
Available from 2018-10-01 10:40

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Dyrhage, Karl
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