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Metabolomic Quality Assessment of EDTA Plasma and Serum Samples
Umea Univ, Swedish Univ Agr Sci, Dept Forest Genet & Plant Physiol, Swedish Metabol Ctr, Umea, Sweden..
Karolinska Inst, Dept Med Epidemiol & Biostat, Nobels Vag 12A, SE-17177 Stockholm, Sweden..
Umea Univ, Swedish Univ Agr Sci, Dept Forest Genet & Plant Physiol, Swedish Metabol Ctr, Umea, Sweden..
Karolinska Univ Hosp, Dept Lab Med, Clin Chem, Stockholm, Sweden..
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2016 (English)In: Biopreservation and Biobanking, ISSN 1947-5535, E-ISSN 1947-5543, Vol. 14, no 5, p. 416-423Article in journal (Refereed) Published
Abstract [en]

Handling and processing of blood can significantly alter the molecular composition and consistency of biobank samples and can have a major impact on the identification of biomarkers. It is thus crucial to identify tools to determine the quality of samples to be used in biomarker discovery studies. In this study, a non-targeted gas chromatography/time-of-flight mass spectrometry (GC-TOFMS) metabolomic strategy was used with the aim of identifying quality markers for serum and plasma biobank collections lacking proper documentation of preanalytical handling. The effect of postcentrifugation delay was examined in serum stored in tubes with gel separation plugs and ethylenediaminetetraacetic acid (EDTA) plasma in tubes with or without gel separation plugs. The change in metabolic pattern was negligible in all sample types processed within 3 hours after centrifugation regardless of whether the samples were kept at 4 degrees C or 22 degrees C. After 8 and 24 hours postcentrifugation delay before aliquoting, there was a pronounced increase in the number of affected metabolites, as well as in the magnitude of the observed changes. No protective effect on the metabolites was observed in gel-separated EDTA plasma samples. In a separate series of experiments, lactate and glucose levels were determined in plasma to estimate the effect of precentrifugation delay. This separate experiment indicates that the lactate to glucose ratio may serve as a marker to identify samples with delayed time to centrifugation. Although our data from the untargeted GC-TOFMS analysis did not identify any specific markers, we conclude that plasma and serum metabolic profiles remain quite stable when plasma and serum are centrifuged and separated from the blood cells within 3 hours.

Place, publisher, year, edition, pages
2016. Vol. 14, no 5, p. 416-423
Keyword [en]
delayed processing of serum, plasma, metabolomics
National Category
Biomedical Laboratory Science/Technology Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-308925DOI: 10.1089/bio.2015.0092ISI: 000386473600009PubMedID: 27348730OAI: oai:DiVA.org:uu-308925DiVA: diva2:1051164
Funder
Swedish Research Council, 829-2009-6285EU, FP7, Seventh Framework Programme, 313010Knut and Alice Wallenberg Foundation
Available from: 2016-12-01 Created: 2016-12-01 Last updated: 2018-01-13Bibliographically approved

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Galli, Joakim

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