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MALDI mass spectrometry imaging of dopamine and PET D1 and D2 receptor ligands in rodent brain tissues
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2015 (English)In: Dopamine Receptor Technologies, Springer-Verlag New York, 2015, 177-196 p.Chapter in book (Refereed)
Abstract [en]

Both pharmaceutical and neurobiological research require a molecular understanding of the complex biochemistry occurring in the brain at a molecular level. To date, this has relied on indirect measurement of labelled compounds or by sample homogenization and subsequent analysis. However, recent advancements in the field of mass spectrometry imaging (MSI) now enabled the direct analysis of molecules from tissue sections. Drugs and endogenous compounds can be simultaneously desorbed/ionized and their abundance measured and mapped across a tissue section, in a multiplexed way. The technologies allow near cellular spatial resolution analysis and quantitative data to be collected. Sample preparation is a crucial step for successful target analyte detection. The use of standard solvent based and novel solvent-free MALDI matrix application methods have been reported as effective for label-free detection of D1 and D2 dopamine receptor antagonists. Furthermore, recently published protocols describe how neurotransmitters previously undetectable directly by MSI can be successfully analyzed following on-tissue derivatization. Mass spectrometry imaging is becoming established as a significant tool for neuroscience and pharmaceutical research and development.

Place, publisher, year, edition, pages
Springer-Verlag New York, 2015. 177-196 p.
Keyword [en]
Derivatization, Dopamine, Dry matrix, Imaging, Label-free, MALDI, Mass spectrometry imaging, Neurotransmitter, PET ligand, Raclopride
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-307077DOI: 10.1007/978-1-4939-2196-6_10ScopusID: 2-s2.0-84955402125ISBN: 9781493921966ISBN: 9781493921959OAI: oai:DiVA.org:uu-307077DiVA: diva2:1051393
Available from: 2016-12-01 Created: 2016-11-08 Last updated: 2016-12-01Bibliographically approved

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Shariatgorji, MohammadrezaAndren, Per E.
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