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Size characterization of green fluorescent protein inclusion bodies in E. coli using asymmetrical flow field-flow fractionation–multi-angle light scattering
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Department of Physical and Analytical Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Department of Physical and Analytical Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
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2006 (English)In: J. of Chromatography A, Vol. 1120, no 1-2, 158-164 p.Article in journal (Refereed) Published
Abstract [en]

The goal of this study was to investigate the applicability of asymmetrical flow field-flow fractionation–multi-angle light scattering (AsFlFFF-MALS) for size analysis of green fluorescent protein inclusion bodies (GFPIBs). The size distributions of GFPIBs prepared by various culture conditions were determined. For GFPIBs prepared at 37 °C the peak maximum hydrodynamic diameter (dH) first increased and then decreased with the increase of the induction times in the presence of 0.1 and 2 mM isopropyl-β-d-thiogalactoside (IPTG). For GFPIBs prepared at 30 °C the peak maximum dH was constant at about 700 nm irrespectively of the induction times and IPTG concentrations.

Place, publisher, year, edition, pages
2006. Vol. 1120, no 1-2, 158-164 p.
Keyword [en]
Asymmetrical flow field-flow fractionation, Multi-angle light scattering, Root mean square radius, Hydrodynamic diameter, Green fluorescence protein inclusion bodies
Identifiers
URN: urn:nbn:se:uu:diva-77550OAI: oai:DiVA.org:uu-77550DiVA: diva2:105462
Available from: 2007-02-14 Created: 2007-02-14 Last updated: 2011-01-11

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Ballagi, AndrasJanson, Jan-Christer

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