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Invisible Analyte Peak Deformations in Single-Component Liquid Chromatography
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology. Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology.
2006 (English)In: Analytical Chemistry, Vol. 78, no 8, p. 2765-2771Article in journal (Refereed) Published
Abstract [en]

It is well known that if a small excess of solute is injected into a chromatographic system equilibrated with an eluent containing the same solute, a single so-called perturbation peak will appear in the chromatogram. It was recently shown (Samuelsson, J.; Forssén, P.; Stefansson, M.; Fornstedt, T. Anal. Chem. 2004, 76, 953-958) that this peak consists of displaced plateau molecules; the injected molecules (mass peak) elute later, together with a deficiency of plateau molecules and are therefore not detected. In this article, we investigated what happens if a large rather than a small excess of solute molecules is injected. To study this systematically, the experimental method involved an enantiomer pair in an achiral separation system. It was found that the invisible mass peak was extremely deformed and that its shape depended on the amount of excess injected, the eluent concentration, and the column length. Depending on these operational conditions, the mass peak changed from a classical Langmuirian (tailing) to an anti-Langmuirian (leading) shape, with deformed shapes observable in the transition. The visible, overloaded perturbation peak was always Langmuirian, regardless of the mass peak shape.

Place, publisher, year, edition, pages
2006. Vol. 78, no 8, p. 2765-2771
Identifiers
URN: urn:nbn:se:uu:diva-77557OAI: oai:DiVA.org:uu-77557DiVA, id: diva2:105469
Available from: 2007-02-13 Created: 2007-02-13 Last updated: 2018-01-23

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Samuelsson, JörgenArnell, RobertFornstedt, Torgny

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