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Analysis of somatropin by double-injection capillary-zone electrophoresis in polybrene/chondroitin sulfate a double-coated capillaries
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
2016 (English)In: Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols / [ed] Nguyet Thuy Tran, Myriam Taverna, New York: Springer-Verlag New York, 2016, p. 93-105Chapter in book (Refereed)
Abstract [en]

Purity determination of somatropin as a recombinant protein is important to ensure its safety and quality. This is carried out by capillary zone electrophoresis in double-injection mode using polybrene/chondroitin sulfate A double-coated capillaries. Modification of the capillary wall eliminates protein-wall interactions which results in improved accuracy and precision of the determinations. In the double-injection mode two somatropin samples are analyzed within a single electrophoretic run. Prior to the second injection, the first injected plug is electrophoresed for a predetermined time period in order to adjust the inter- plug distance. Here, the principle for the separation of somatropin charge variants is described.

Place, publisher, year, edition, pages
New York: Springer-Verlag New York, 2016. p. 93-105
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1466
Keywords [en]
Double-injection capillary-zone electrophoresis (DICZE), Partial electrophoresis time period (tPE), Polybrene/ chondroitin sulfate A (PB/CA) double-coated capillary, Somatropin
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-310283DOI: 10.1007/978-1-4939-4014-1_8Scopus ID: 2-s2.0-84990242861ISBN: 978-1-4939-4012-7 (print)ISBN: 978-1-4939-4014-1 (print)OAI: oai:DiVA.org:uu-310283DiVA, id: diva2:1056903
Available from: 2016-12-15 Created: 2016-12-13 Last updated: 2018-01-13Bibliographically approved

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Amini, Ahmad

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