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Sensitive magnetic biodetection using magnetic multi-core nanoparticles and RCA coils
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2016 (English)In: Journal of Magnetism and Magnetic Materials, ISSN 0304-8853, E-ISSN 1873-4766, Vol. 427, p. 14-18Article in journal (Refereed) Published
Abstract [en]

We use functionalized iron oxide magnetic multi-core particles of 100 nm in size (hydrodynamic particle diameter) and AC susceptometry (ACS) methods to measure the binding reactions between the magnetic nanoparticles (MNPs) and bio-analyte products produced from DNA segments using the rolling circle amplification (RCA) method. We use sensitive induction detection techniques in order to measure the ACS response. The DNA is amplified via RCA to generate RCA coils with a specific size that is dependent on the amplification time. After about 75 min of amplification we obtain an average RCA coil diameter of about 1 µm. We determine a theoretical limit of detection (LOD) in the range of 11 attomole (corresponding to an analyte concentration of 55 fM for a sample volume of 200 µL) from the ACS dynamic response after the MNPs have bound to the RCA coils and the measured ACS readout noise. We also discuss further possible improvements of the LOD.

Place, publisher, year, edition, pages
2016. Vol. 427, p. 14-18
Keywords [en]
Magnetic nanoparticles, Magnetic multi-core particles, AC susceptometry, RCA coil, Differential induction coil, Biodetection
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-310469DOI: 10.1016/j.jmmm.2016.10.041ISI: 000397199400004OAI: oai:DiVA.org:uu-310469DiVA, id: diva2:1057036
Funder
Knut and Alice Wallenberg FoundationAvailable from: 2016-12-16 Created: 2016-12-16 Last updated: 2017-05-30

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Zardán Gómez de la Torre, TeresaStrømme, Maria

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