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Hyperelastic Nanocellulose-Reinforced Hydrogel of High Water Content for Ophthalmic Applications
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanotechnology and functional materials)ORCID iD: 0000-0001-7638-0925
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.ORCID iD: 0000-0003-2079-4170
Department of Clinical and Experimental Medicine, Linköping University, 581 85 Linköping, Sweden..
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2016 (English)In: ACS Biomaterials Science and Engineering, ISSN 2373-9878, Vol. 2, no 11, p. 2072-2079Article in journal (Refereed) Published
Abstract [en]

A nanocellulose-reinforced poly(vinyl alcohol) hydrogel material of exceptionally high water content for ophthalmic applications is presented (>90 wt %), which also features a hitherto unprecedented combination of optical, mechanical, viscoelastic, oxygen permeability, and biocompatibility properties. The hydrogel combines the desired softness with remarkable strain-dependent mechanical strength and thereby demonstrates hyperelastic, rubber-like mechanical properties. The observed unusual mechanical behavior is due to both high water content and the combination of relatively stiff cellulose nanowhiskers entangled in a soft polymer matrix of poly(vinyl alcohol) (PVA), thus mimicking the structural characteristics of the cornea's main constituents, i.e., water and collagen.

Place, publisher, year, edition, pages
2016. Vol. 2, no 11, p. 2072-2079
Keyword [en]
biocompatibility, cellulose nanocrystals, contact lens, ophthalmic prosthesis, poly(vinyl alcohol)
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
URN: urn:nbn:se:uu:diva-310474DOI: 10.1021/acsbiomaterials.6b00484ISI: 000388161200027Scopus ID: 2-s2.0-84995379882OAI: oai:DiVA.org:uu-310474DiVA, id: diva2:1057044
Projects
WWSC
Funder
Knut and Alice Wallenberg FoundationVINNOVA, 2013-04645
Available from: 2016-12-16 Created: 2016-12-16 Last updated: 2018-03-19Bibliographically approved
In thesis
1. Hydrogels of Poly(vinyl alcohol) and Nanocellulose for Ophthalmic Applications: Synthesis, Characterization, Biocompatibility and Drug Delivery Studies
Open this publication in new window or tab >>Hydrogels of Poly(vinyl alcohol) and Nanocellulose for Ophthalmic Applications: Synthesis, Characterization, Biocompatibility and Drug Delivery Studies
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Hydrogels are commonly used materials in ophthalmic care as contact lenses, bandage lenses, corneal implants, and cornea regeneration scaffolds. Hydrogels can be produced by physical, chemical, or radiation crosslinking of hydrophilic polymers. Poly(vinyl alcohol) (PVA) is a hydrophilic polymer that has been long known to the scientific community and is used in ophthalmic formulations.

In this thesis, PVA was reinforced with nanocellulose to obtain self-standing hydrogels. Cryo-gelation technique was used to obtain transparent hydrogels from PVA dissolved in DMSO-water mixed solvent. The properties of these hydrogels were analyzed to explore the possibility of their application for ophthalmic use as a drug delivery vehicle and as cornea regeneration implant.

The results indicate that oxidized nanocellulose can be combined with PVA to produce transparent, elastic, macroporous and high-water content hydrogel lenses. The water-filled macroporous structure of these hydrogels aids with oxygen transport and can enhance comfort while worn. The developed hydrogel also features moderate UV-light blocking properties in addition to high transparency. Furthermore, it was observed that the light scattering due to surface roughness of the hydrogel increases with measurement time, due to the rapid evaporation of the water layer from the surface of the hydrogel film.

Mechanical analysis results revealed that the hydrogels exhibited a strain-induced stiffening behavior, which is usually noticed in hyper-elastic materials and collagenous soft tissues. The results of this study suggest that in order to mimic collagenous behavior, the material should possess high water content and a specific structural architecture combining soft and rigid elements as building blocks.

Furthermore, PVA-CNC composite hydrogel showed no toxic effects on the corneal cells in both direct and indirect contact studies. It was found that the hydrogel promoted cell attachment and was stable when sutured ex vivo to a porcine excised cornea.

To study enzyme-triggered drug release, hydrogel lenses loaded with chitosan-poly(acrylic acid) nanoparticles were exposed to lysozyme, an enzyme present in the eye. Nanoparticles were shown to disintegrate due to the hydrolysis of chitosan chains by lysozyme. Overall, with these distinctive properties, PVA-CNC hydrogel has great potential as an ophthalmic biomaterial for therapeutic and cornea regeneration applications. 

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2018. p. 76
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1650
Keyword
nanocellulose, poly(vinyl alcohol), hydrogel, cornea regeneration, therapeutic lens
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
Identifiers
urn:nbn:se:uu:diva-346478 (URN)978-91-513-0285-0 (ISBN)
Public defence
2018-05-17, Polhemssalen, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 09:30 (English)
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Supervisors
Available from: 2018-04-17 Created: 2018-03-19 Last updated: 2018-04-17

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Tummala, Gopi KrishnaJoffre, ThomasLopes, VivianaFerraz, NataliaPersson, CeciliaMihranyan, Albert

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