Titanium surface modification leading to increased antibacterial ability, bioactivity and biocompatibility
2016 (English)Conference paper, Presentation (Refereed)
Bacterial biofilms can adhere to implants, and may ultimately lead to implant failure. One way to prevent bacterial biofilm formation is to modify the surface to make it antibacterial or bacteriostatic. A straightforward and economically attractive modification method is to soak the surface in hydrogen peroxide, sodium hydroxide and calcium dihyroxide. Hydrogen peroxide has been found to make the surface anti-inflammatory and perhaps antibacterial. Sodium hydroxide has been seen to render the surface bioactive, while calcium hydroxide might further increase the bioactivity. In this study we investigated the antibacterial properties, bioactivity and biocompatibility of this surface modification method.
Coupons of cpTi (grade 2) were immersed in H2O2 for 1 h at 80 °C and then soaked in NaOH. Some test groups were also soaked in Ca(OH)2. After soaking, coupons were heat treated at 200 °C, autoclaved at 125 °C for 1 h or simply kept at room temperature. To investigate bioactivity the coupons were immersed in SBF for 7 days. Biocompatibility was assessed by seeding two cell lines on the modified titanium surfaces. To investigate the antibacterial effect, bacterial biofilms were grown on the surfaces for 16 h and assessed for viability with luminescence readings.
Ca(OH)2 modified coupons showed an increased bioactivity compared to coupons only soaked in NaOH. Hydroxyapatite formation was strongest for test groups placed in room temperature or 200 °C. Cell proliferation was increased with human dermal fibroblast. Autoclaved surfaces showed a decreased luminescence signal compared to the control, indicating inhibition of bacterial biofilm.
Coupons soaked in Ca(OH)2 after soaking in NaOH showed increased bioactivity compared to coupons only soaked in NaOH. Further they exhibit excellent biocompatibility and some degree of antibacterial behavior.
 P. Tengvall, I. Lundström, L. Sjöqvist, H. Elwing, L.M. Bjursten, Biomaterials 10 (1989) 166.
 X. LIU, P. CHU, C. DING, Materials Science and Engineering: R: Reports 47 (2004) 49.
Place, publisher, year, edition, pages
Engineering and Technology
Research subject Engineering Science with specialization in Materials Science
IdentifiersURN: urn:nbn:se:uu:diva-310502OAI: oai:DiVA.org:uu-310502DiVA: diva2:1057130
BIOMATERIALS for Tissue engineering Models - Reykjavík, Iceland