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Initiation of Escherichia coli ribosomes on matrix coupled mRNAs studied by optical biosensor technique
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology.
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1999 (English)In: BIOCHIMIE, ISSN 0300-9084, Vol. 81, no 10, 995-1002 p.Article in journal (Other scientific) Published
Abstract [en]

The optical biosensor technique, based on the surface plasmon resonance (SPR) phenomenon, has been used to study the initiation of protein synthesis by E. coli ribosomes on surface coupled mRNA. mRNA was first periodate oxidized and then hydrazide coupled

Place, publisher, year, edition, pages
EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER , 1999. Vol. 81, no 10, 995-1002 p.
Keyword [en]
in vitro protein synthesis; initiation; BIACORE; solid phase translation; SURFACE-PLASMON RESONANCE; MESSENGER-RNA TRANSLATION; PROTEIN-SYNTHESIS; SELECTION; SYSTEM; REGION; SITE; PURIFICATION; TECHNOLOGY; BINDING
Identifiers
URN: urn:nbn:se:uu:diva-77967OAI: oai:DiVA.org:uu-77967DiVA: diva2:105880
Note
Addresses: Ehrenberg M, BMC, Dept Cell & Mol Biol, Box 596, S-75124 Uppsala, Sweden. BMC, Dept Cell & Mol Biol, S-75124 Uppsala, Sweden. Biacore AB, S-75450 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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