uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Atomic force microscopy and direct surface force measurements
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Department of Physical and Analytical Chemistry, Surface Biotechnology.
Show others and affiliations
2005 (English)In: Pure and Applied Chemistry, ISSN 0033-4545, Vol. 77, no 12, 2149–2170- p.Article in journal (Refereed) Published
Abstract [en]

IUPAC Physical and Biophysical Chemistry Division

The atomic force microscope (AFM) is designed to provide high-resolution (in the ideal case, atomic) topographical analysis, applicable to both conducting and nonconducting surfaces. The basic imaging principle is very simple: a sample attached to a piezoelectric positioner is rastered beneath a sharp tip attached to a sensitive cantilever spring. Undulations in the surface lead to deflection of the spring, which is monitored optically. Usually, a feedback loop is employed, which holds the spring deflection constant, and the corresponding movement of the piezoelectric positioner thus generates the image. From this it can be seen that the scanning AFM has all the attributes necessary for the determination of surface and adhesion forces; a sensitive spring to determine the force, a piezoelectric crystal to alter the separation of the tip and surface, which if sufficiently well-calibrated also allows the relative separation of the tip and surface to be calculated. One can routinely quantify both the net surface force (and its separation dependence) as the probe approaches the sample, and any adhesion (pull-off) force on retraction. Interactions in relevant or practical systems may be studied, and, in such cases, a distinct advantage of the AFM technique is that a particle of interest can be attached to the end of the cantilever and the interaction with a sample of choice can be studied, a method often referred to as colloid probe microscopy. The AFM, or, more correctly, the scanning probe microscope, can thus be used to measure surface and frictional forces, the two foci of this article. There have been a wealth of force and friction measurements performed between an AFM tip and a surface, and many of the calibration and analysis issues are identical to those necessary for colloid probe work. We emphasize that this article confines itself primarily to elements of colloid probe measurement using the AFM.

Place, publisher, year, edition, pages
2005. Vol. 77, no 12, 2149–2170- p.
Keyword [en]
AFM, atomic force, colloids, colloid probes, IUPAC Physical and Biophysical Chemistry Division
URN: urn:nbn:se:uu:diva-78099OAI: oai:DiVA.org:uu-78099DiVA: diva2:106012
Available from: 2006-03-17 Created: 2006-03-17 Last updated: 2011-01-11

Open Access in DiVA

No full text

By organisation
Surface BiotechnologySurface Biotechnology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 176 hits
ReferencesLink to record
Permanent link

Direct link