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Forensic casework analysis using the HVI/HVII mtDNA linear array assay.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Biology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Biology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Genetics and Pathology.
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2005 (English)In: Journal of Forensic Sciences, ISSN 0022-1198, E-ISSN 1556-4029, Vol. 50, no 3, 548-554 p.Article in journal (Refereed) Published
Abstract [en]

The mitochondrial hypervariable regions I and II have proven to be a useful target for analysis of forensic materials, in which the amount of DNA is limited or highly degraded. Conventional mitochondrial DNA (mtDNA) sequencing can be time-consuming and expensive, limitations that can be minimized using a faster and less expensive typing assay. We have evaluated the exclusion capacity of the linear array mtDNA HVI/HVII region-sequence typing assay (Roche Applied Science) in 16 forensic cases comprising 90 samples. Using the HVI/HVII mtDNA linear array, 56% of the samples were excluded and thus less than half of the samples require further sequencing due to a match or inconclusive results. Of all the samples that were excluded by sequence analysis, 79% could be excluded using the HVI/HVII linear array alone. Using the HVI/HVII mtDNA linear array assay, we demonstrate the potential to decrease sequencing efforts substantially and thereby reduce the cost and the turn-around time in casework analysis.

Place, publisher, year, edition, pages
2005. Vol. 50, no 3, 548-554 p.
Keyword [en]
Crime, DNA primers, DNA, mitochondrial/*analysis, female, forensic medicine/methods, hair/*chemistry, humans, male, oligonucleotide array sequence analysis/*methods, research support, non-U.S. Gov't, research support, U.S. Gov't, non-P.H.S., sequence analysis, DNA/*methods
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Forensic Science
Identifiers
URN: urn:nbn:se:uu:diva-78183ISI: 000228818800009PubMedID: 15932085OAI: oai:DiVA.org:uu-78183DiVA: diva2:106096
Available from: 2006-03-20 Created: 2009-03-26 Last updated: 2017-12-14Bibliographically approved

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Divne, Anna-MariaNilsson, MartinaAllen, Marie

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Molecular BiologyDepartment of Immunology, Genetics and PathologyDepartment of Genetics and Pathology
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