Posttranscriptional regulation in adenovirus infected cells
2016 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907Article in journal (Refereed) Published
A deeper understanding of how viruses reprogramtheir hosts for production of progeny is needed to combatinfections. Most knowledge on the regulation of cellular geneexpression during adenovirus infection is derived from mRNAstudies. Here, we investigated the changes in protein expressionduring the late phase of adenovirus type 2 (Ad2) infection of theIMR-90 cell line by stable isotope labeling in cell culture withsubsequent liquid chromatography−high resolution tandemmass spectrometric analysis. Two biological replicates of samplescollected at 24 and 36 h post-infection (hpi) were investigated using swapped labeling. In total, 2648 and 2394 proteins werequantified at 24 and 36 hpi, respectively. Among them, 659 and 645 were deregulated >1.6-fold at the two time points. Theprotein expression was compared with RNA expression using cDNA sequencing data. The correlation was surprisingly low(r = 0.3), and several examples of posttranscriptional regulation were observed; e.g., proteins related to carbohydrate metabolismwere up-regulated at the protein level but unchanged at the RNA level, whereas histone proteins were down-regulated at theprotein level but up-regulated at the RNA level. The deregulation of cellular gene expression by adenovirus is mediated atmultiple levels and more complex than hitherto believed.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2016.
Analytical Chemistry Cell and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-311796DOI: 10.1021/acs.jproteome.6b00834OAI: oai:DiVA.org:uu-311796DiVA: diva2:1061495
FunderCarl Tryggers foundation Åke Wiberg FoundationMagnus Bergvall Foundation