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Sulfonation chemistry as a powerful tool for MALDI TOF/TOF de novo sequencing and post-translational modification analysis
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
2005 (English)In: Journal of biomolecular techniques, ISSN 1524-0215, Vol. 16, no 4, 441-452 p.Article in journal (Refereed) Published
Abstract [en]

Mass spectrometry using matrix-assisted laser desorption/ionization (MALDI) is a widespread technique for various types of proteomic analysis. In the identification of proteins using peptide mass fingerprinting, samples are enzymatically digested and resolved into a number of peptides, whose masses are determined and matched with a sequence data-base. However, the presence inside the cell of several splicing variants, protein isoforms, or fusion proteins gives rise to a complex picture, demanding more complete analysis. Moreover, the study of species with yet uncharacterized genomes or the investigation of post-translational modifications are not possible with classical mass fingerprinting, and require specific and accurate de novo sequencing. In the last several years, much effort has been made to improve the performance of peptide sequencing with MALDI. Here we present applications using a fast and robust chemical modification of peptides for improved de novo sequencing. Post-source decay of derivatized peptides generates at the same time peaks with high intensity and simple spectra, leading to a very easy and clear sequence determination.

Place, publisher, year, edition, pages
2005. Vol. 16, no 4, 441-452 p.
National Category
Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-78720PubMedID: 16522867OAI: oai:DiVA.org:uu-78720DiVA: diva2:106633
Available from: 2006-03-29 Created: 2006-03-29 Last updated: 2011-02-28Bibliographically approved

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Conrotto, PaoloHellman, Ulf

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