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A direct estimation of the context effect on the efficiency of termination
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology.
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1998 (English)In: JOURNAL OF MOLECULAR BIOLOGY, ISSN 0022-2836, Vol. 284, no 3, 579-590 p.Article in journal (Other scientific) Published
Abstract [en]

An in vitro assay in which terminating Escherichia coli ribosomes with different stop signals in the A-site compete for a limited amount of a release factor (RF1 or RF2) has been used to estimate the relative termination efficiencies at stop codons with d

Place, publisher, year, edition, pages
ACADEMIC PRESS LTD , 1998. Vol. 284, no 3, 579-590 p.
Keyword [en]
context dependence; release factors; in vitro translation; role of RF3; RECYCLING FACTOR RRF; RELEASE FACTOR RF3; ESCHERICHIA-COLI; TRANSFER-RNA; TRANSLATION TERMINATION; NASCENT PEPTIDE; AMINO-ACID; RIBOSOME; CODON; PURIFICATION
Identifiers
URN: urn:nbn:se:uu:diva-79096OAI: oai:DiVA.org:uu-79096DiVA: diva2:107009
Note
Addresses: Pavlov MY, Biomed Ctr, Dept Mol Biol, Box 590, S-75124 Uppsala, Sweden. Biomed Ctr, Dept Mol Biol, S-75124 Uppsala, Sweden. Inst Biol Physicochim, CNRS, UPR9073, F-75005 Paris, France.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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