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Synthesis of region-labelled proteins for NMR studies by in vitro translation of column-coupled mRNAs
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology.
1997 (English)In: BIOCHIMIE, ISSN 0300-9084, Vol. 79, no 7, 415-422 p.Article in journal (Other scientific) Published
Abstract [en]

A method to synthesise region-labelled proteins for structural studies with NMR is suggested. The technique is based on in vitro translation of matrix-coupled mRNAs. Translation starts with unlabelled amino acids from the initiation codon of the mRNA and

Place, publisher, year, edition, pages
EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER , 1997. Vol. 79, no 7, 415-422 p.
Keyword [en]
NMR protein structure; gene expression; in vitro translation; protein labelling; NUCLEAR-MAGNETIC-RESONANCE; DNA-BINDING DOMAIN; ESCHERICHIA-COLI; TRP REPRESSOR; SPECTROSCOPY; PURIFICATION; INITIATION; ELONGATION; RNA
Identifiers
URN: urn:nbn:se:uu:diva-79451OAI: oai:DiVA.org:uu-79451DiVA: diva2:107364
Note
Addresses: Pavlov MY, BMC, DEPT MOL BIOL, BOX 590, S-75124 UPPSALA, SWEDEN.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-15

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