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The "PepSAVI-MS" Pipeline for Natural Product Bioactive Peptide Discovery
Univ North Carolina Chapel Hill, Dept Chem, Chapel Hill, NC USA..
Univ North Carolina Chapel Hill, Dept Chem, Chapel Hill, NC USA..
Univ North Carolina Chapel Hill, Dept Chem, Chapel Hill, NC USA..
Univ North Carolina Chapel Hill, Dept Chem, Chapel Hill, NC USA..
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2017 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 89, no 2, 1194-1201 p.Article in journal (Refereed) Published
Abstract [en]

The recent increase in extensively drug-resistant bacterial pathogens and the associated increase of morbidity and mortality demonstrate the immediate need for new antibiotic backbones with novel mechanisms of action. Here, we report the development of the PepSAVI-MS pipeline for bioactive peptide discovery. This highly versatile platform employs mass spectrometry and statistics to identify bioactive peptide targets from complex biological samples. We validate the use of this platform through the successful identification of known bioactive peptides from a botanical species, Viola odorata. Using this pipeline, we have widened the known antimicrobial spectrum for V. odorata cyclotides, including antibacterial activity of cycloviolacin O2 against A. baumannii. We further demonstrate the broad applicability of the platform through the identification of novel anticancer activities for cycloviolacins by their cytotoxicity against ovarian, breast, and prostate cancer cell lines.

Place, publisher, year, edition, pages
2017. Vol. 89, no 2, 1194-1201 p.
National Category
Analytical Chemistry Medicinal Chemistry
Identifiers
URN: urn:nbn:se:uu:diva-316030DOI: 10.1021/acs.analchem.6b03625ISI: 000392458100025OAI: oai:DiVA.org:uu-316030DiVA: diva2:1076893
Available from: 2017-02-24 Created: 2017-02-24 Last updated: 2017-03-28

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Strömstedt, Adam A.Göransson, Ulf
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