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Thermodynamic and kinetic approaches for evaluation of monoclonal antibody - Lipoprotein interactions
Univ Helsinki, Dept Chem, Analyt Chem Lab, POB 55, FI-00014 Helsinki, Finland..
Univ Helsinki, Dept Chem, Analyt Chem Lab, POB 55, FI-00014 Helsinki, Finland..
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Medical Radiation Science. Ridgeview Instruments AB, SE-74020 Vange, Sweden..
Karlstad Univ, Dept Engn & Chem Sci, SE-65188 Karlstad, Sweden..
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2017 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 518, 25-34 p.Article in journal (Refereed) Published
Abstract [en]

Two complementary instrumental techniques were used, and the data generated was processed with advanced numerical tools to investigate the interactions between anti-human apoB-100 monoclonal antibody (anti-apoB-100 Mab) and apoB-100 containing lipoproteins. Partial Filling Affinity Capillary Electrophoresis (PF-ACE) combined with Adsorption Energy Distribution (AED) calculations provided information on the heterogeneity of the interactions without any a priori model assumptions. The AED calculations evidenced a homogenous binding site distribution for the interactions. Quartz Crystal Microbalance (QCM) studies were used to evaluate thermodynamics and kinetics of the Low-Density Lipoprotein (LDL) and anti-apoB-100 Mab interactions. High affinity and selectivity were observed, and the emerging data sets were analysed with so called Interaction Maps. In thermodynamic studies, the interaction between LDL and anti-apoB-100 Mab was found to be predominantly enthalpy driven. Both techniques were also used to study antibody interactions with Intermediate-Density (IDL) and Very Low Density (VLDL) Lipoproteins. By screening affinity constants for IDL-VLDL sample in a single injection we were able to distinguish affinity constants for both subpopulations using the numerical Interaction Map tool.

Place, publisher, year, edition, pages
2017. Vol. 518, 25-34 p.
Keyword [en]
Monoclonal anti-apoB-100 antibody, Lipoproteins, Thermodynamics, Kinetics, Interaction map
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-316011DOI: 10.1016/j.ab.2016.10.024ISI: 000392461000004PubMedID: 27984014OAI: oai:DiVA.org:uu-316011DiVA: diva2:1076976
Funder
Swedish Research Council, 2015-04627
Available from: 2017-02-24 Created: 2017-02-24 Last updated: 2017-02-24Bibliographically approved

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